THE ROLE OF HUMORAL FACTORS IN THE MECHANISMS OF GRAFT-VERSUS­ HOST INDUCED HOST CELL PROLIFERATION AND LYMPH NODE HYPERPLASIA IN THE RAT

B Rolstad

Abstract


The effect of humoral factors relea ed during ystemic
graft-versu -host (GVH) reactions on cell proliferation
and lymph node enlargement was investigated
as follows:
1) Thoracic duct lymphocytes:of host origin were
cultured in diffusion chambers in the peritoneal cavity
of rats undergoing a systemic GVI-I reaction.
On day 7 of the response chamber cell proliferation
(as judged by 31-1 thymidine incorporation)
was increased two to four fold over that seen in
normal hosts. 2) Serum from (HOxDA) F 1 hybrid
rats being repeatedly injected with parental (HO)
cells contained a factor that, when preincubatcd
with such parental cells in vitro, was able to augment
their ability to produce lymph node enlargement
in a local GVH reaction. These results suggest
that the host cell proliferation and lymphoid hyperplasia
observed during GVH reactions might be the
results of long range humoral mechanisms acting
alone or in addition to the direct interaction between
donor and host cells.


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