EFFECT OF LOW MOLECULAR FACTORS RELEASED DURING ALLOGENEIC INTERACTIONS OF LEUCOCYTES OR BRAIN CELLS ON PHA- AND LPS-INDUCED DNA SYNTHESIS IN LYMPHOCYTES

H Kovaru, M Pospisil

Abstract


Male (A/Ph and C3H/Cbi/BOM) mice were used in
our experiments. The cell-free supernatants of spleen
mixed leucocyte cultures and allogeneic brain cortex
cells were collected upon short-term incubation and
tested in a comparative study. Spleen and brain low
molecular factors were fractionated (ultrafiltration
and gel column cluomatography) and assayed for
their activity in mitogen-induced 3H-thymidine incorporat
ion into spleen lymphocytes.
The specific release of the allogeneic spleen fraction
(ASF-2) has been observed. A non-specific stimulating
effect of ASF-2 on PHA- and LPS-activated
spleen lymphocytes was shown. In contrast, a nonpecific
suppress ive effect of brain fraction on PHAand
LPS-stimulated lymphocytes was demonstrated.
The opposite effects of spleen and brain factors can
be connected with their different composition.
The lymphocyte cultures were not affected by spleen
or brain factor, when cultured without the addition
of mitogen.
Positive and negative regulation or modulation of
triggering events in lymphocyte activation by these
factors can be suggested.


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