INFLUENCE OF A LYMPHAGOGUE, CLS 2210, ON REGIONAL CARDIAC LYMPHATICS AND THE ELECTROCARDIOGRAM AFTER CORONARY ARTERY OCCLUSION IN THE DOG

Abstract

To examine the role of cardiac lymphatic
drainage in myocardial infarction, we quantified
the effect of a lymphogogue, CLS 2210, on the
number and appearance of myocardial lymphatics
as well as the electrocardiogram following
coronary occlusion in the dog. Thirty minutes
and six hours after intravenous administration of
the benzenesulfonate compound, (CLS 2210)
cardiac lymphatics in the distribution of the left
anterior descending coronary artery (LAD) were
determined and further delineated by
postmortem cardiac lymphangiograms. The
results were compared with treated and untreated
dogs without and with descending coronary
artery ligation including the non infarcted
zone; that is, myocardium within the distribution
of left circumflex coronary (LCC) artery. After
30 minutes in dogs receiving CLS 2210 without
LAD ligation, number of lymphatics (point
countlcmZ, see Methods) were respectively -
LAD zone: 2.62 ± 0.11 or 10.9% of left ventricular
(LV) surface; LCC zone: 2.87 ± 0.10,
whereas after six hours-LAD zone 8.04 ±
0.03 or 32.3% LV surface; LCC zone-8.13 ±
0.06 compared with untreated controls-LAD
zone 1. 71 ± 0.11 or 6.6% of LV surface; LCC
zone 1.65 ± 0.12 (p < 0.0001). At similar intervals
in dogs with LAD ligation, the findings
were at 30 minutes LAD zone 0.78 ± 0.07 or
3.1 % of LV surface and at 360 'minutes was
0.80 ± 0.08 or 3.3% of LV surface. In conjunction
with CLS 2210 administration, however,
LAD zone showed at 30 minutes 2.50 ± 0.12
or 10% of LV surface (p < .01) and at 360
minutes was 10.34 ± 0.03 or 35.1% of LV surface.
Moreover, in dogs with LAD ligation and
CLS 2210 administration, electrocardiogram at six                                     hours showed diminished ST-segment elevation