THE REGULATORY EFFECTS OF CYTOKINES ON LYMPHATIC ANGIOGENESIS

N-F Liu, Q-L He

Abstract


The effects of fibroblast growth factor basic (bFGF), transforming growth factor " (TGF"),recombinant human epidermal growth factor (EGF), recombinant human tumor necrosis factor" (TNF"), and recombinant interleukin 1" (IL-1") on lymphatic angiogenesis were assessed incultured newborn bovine lymphatic endothelial cells (NBLEC). bFGF, TGF", and EGFstimulated the proliferation of NBLEC in a dose-dependent manner, but the combination ofeither two growth factors did not show synergistic effects on NBLEC DNA synthesis. TNF" andIL-1" suppressed the multiplication of NBLEC. Treatment with bFGF markedly increased themigration of NBLEC. The tissue plasminogen activator (t-PA) activity was enhanced by bFGF.TNF" also promoted NBLEC t-PA activity.These results suggest that bFGF is a major multifunctional lymphatic endothelial celltargeted cytokine, and both growth and pro-inflammatory cytokines exert differential regulatoryeffects on lymphatic endothelial cell proliferation, migration and t-PA activity.

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