EFFECT OF TUMOR NECROSIS FACTOR-" AND INTERLEUKIN-2 ON SPLEEN LYMPHOCYTE MIGRATION IN MOUSE SKIN

Abstract

Tumor necrosis factor-" (TNF-") and interleukin-2 (IL-2) are reported to enhancelymphocyte binding to endothelial cells in vitro. We examined these two agents on lymphocytemigration in vivo. Spleen lymphocytes were radiolabeled with tritiated uridine (3H-UR) and theninjected IV into mice. Each cytokine (TNF-" or IL-2) or both cytokines were then injectedintradermally on the back of mice. The results demonstrated that TNF-" stimulates lymphocytemigration in vivo in dose-dependent fashion. Kinetic analysis demonstrated that migration withTNF-" started at 3h, peaked at 6h, followed by a gradual decline back to baseline at 24h. IL-2,on the other hand, was nearly inactive, and did not augment lymphocyte migration over andabove that induced by TNF-" when both cytokines were injected together.