EICOSANOID PRODUCTION AND LYMPHATIC RESPONSIVENESS IN HUMAN CIGARETTE SMOKERS COMPARED WITH NON-SMOKERS

Abstract

Leg lymphatic segments were isolated from 10 patients (4 cigarette smokers and 6 nonsmokers) undergoing conventional lymphography. Prostaglandin (PG) levels and PG synthesisin the lymphatics and in a variety of body fluids and the effects of eicosanoids on lymphaticcontractility were determined. Leg lymphatics from 4 smokers generated less PGI2 and containedmore 8-epi-PGF2" when compared with leg lymphatics in 6 non-smokers. Similarly, levels of 8-epi-PGF2" in smokers compared with non-smokers were higher in plasma (28.6 cf 19.7 pg/ml),leg lymph (146.7 cf 65.3 pg/ml), serum (299.0 cf 204.1 pg/ml), and urine (473.4 cf 241.0 pg/mgcreatinine). Lymphatics from smokers also showed a higher contractile response, less 14Carachidonic acid conversion to PGI2 and less PGI2-formation with various stimuli compared withnon-smokers. Together these findings suggest that smoking induces oxidation injury, promotesaltered (iso-)eicosanoid production and impacts on the function and dysfunction of peripherallymphatics under normal circumstances and in a variety of clinical disorders.