HEPARIN INHIBITS P388D1 CELLS ADHERENCE AND METASTASIS TO PERIPHERAL LYMPH NODES IN VITRO AND IN VIVO

Abstract

Heparin is known to attenuate tumormetastasis mainly by inhibiting the interactionbetween L-selectin and its ligand. However,the mechanism of heparin on lymphoma isunclear. This report demonstrates that bothL-selectin/h IgG chimeric protein and heparintreatment can significantly inhibit theadhesion of P388D1 cells onto lymphaticsinusoids and marginal sinusoids in vitro, thatheparin can attenuate P388D1 cell homingto lymph nodes in vivo at 12 hours, and thatheparin significantly reduced P388D1 cellsmetastasis to lymph nodes 18 days afterinjection. These results indicate that heparinmay act as a ligand for L-selectin on theP388D1 macrophage-like lymphoma cell lineto attenuate tumor growth and metastasis.